While various chemical ligation methods are available, they are typically limited to short fragments and are often inefficient for long-range RNA ligation or result in the formation of non-canonical (unnatural) linkages within the mRNA [317,318,319]
This dialysis bag was then immersed in either 200 mL of phosphate-buffered saline (PBS) or a 200 mL aqueous solution of hydrogen peroxide (H 2 O 2 ) at a concentration of 10 mM, all while being maintained in a shaking culture incubator at 37 C
OH was supported by MHCZDRO-VFN00064165 and the National Institute for Cancer Research (reg
J Psychiatr Res 121:1023 Lv D, Cui PL, Yao SW, Xu YQ, Yang ZX (2012) Melatonin inhibits the expression of vascular endothelial growth factor in pancreatic cancer cells
Solution left out of the fridge Short excursions at room temperature (under 2 hours) are generally tolerable